Widal Test (Slide Agglutination)

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Widal test (Slide agglutination) is a clinical test used to diagnose enteric fever commonly referred to as typhoid fever.

What is Widal test (Slide agglutination)?

Widal test (Slide agglutination) was developed in 1896 by Georges Ferdinand Widal. Widal test (Slide agglutination) is used to examine the presence of salmonella bacteria in the blood. The antibodies that are produced in response to the infection of salmonella bacteria are used for diagnosis in Widal test (Slide agglutination). [2]

Why is Widal test (Slide agglutination) suggested?

Widal test (Slide agglutination) is suggested if the patient has the following symptoms

  • Fever and Fatigue.
  • Unexplained weight loss and abdominal pain.
  • Rashes, also known as “rose spots”. [1]

How is Widal test (Slide agglutination) performed?

Widal test (Slide agglutination) involves the collection of blood sample from the patient.

  • Antigens are prepared prior to the test and dyes are added for identification.
  • An antigen is added to the blood sample along with positive control and negative control.
  • The reagents are thoroughly mixed with the blood sample.

Formation of agglutination during the Widal test (Slide agglutination) confirms the presence of infection.  [3]

What are the parameters measured in Widal test (Slide agglutination)?

The antibodies produced in blood in response to the infection of salmonella bacteria are measured in the Widal test (Slide agglutination). [2]

Preparation before Widal test (Slide agglutination)

No major preparation is needed for the Widal test (Slide agglutination).

Avoid alcohol and other medications 24 hours prior to Widal test (Slide agglutination).

Post-care after Widal test (Slide agglutination)

No special care is needed after Widal test (Slide agglutination).

  • You can resume your normal activities after Widal test (Slide agglutination). [2]

Sample types in Widal test (Slide agglutination)

A blood sample is usually used in the Widal test (Slide agglutination).

  • Stool, Urine or Bone marrow culture may also be used as a sample. [1]

Risks of Widal test (Slide agglutination)

There are no real risks involved in Widal test (Slide agglutination).

  • A temporary throbbing in the injected area.
  • Other non-enteric infections cause false positive results. [2]

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