PML-RARA is one of the primary tests performed to check the presence of Acute Promyelocytic Leukemia (APL), which is a subtype of Acute Myeloid Leukemia (AML) – a cancer of White Blood Cells.
What is PML-RARA (APML)?
Cancerous cells affected with Acute Promyelocytic Leukemia (APL) have a fused gene. It has a downstream sequence of Retinoic Acid Receptor Alpha (RARA) gene fused with the promoter and upstream gene sequences usually of the Promyelocytic Leukemia (PML) gene. Through the PML-RARA test, the Messenger RNA created from this fused gene is recognized, thereby helping in the detection of the presence of APL.
Why is PML-RARA (APML) suggested?
A PML-RARA (APML) test can be advised in the following cases:
- When the physician suspects that a person has APL.
- When abnormal Complete Blood Count and/or Blood Smear findings, coupled with nonspecific symptoms (which may be related to Leukemia), are present.
How is PML-RARA (APML) performed?
To conduct the PML-RARA (APML) test, a blood or bone marrow sample is obtained from the patient in the following way:
- A blood sample from a vein in the arm is collected.
- In case of a bone marrow sample, the sample is collected mostly from the hip bone.
- For the initial diagnostic steps, both the blood and the bone marrow may be examined. In the follow-up tests for monitoring purposes, however, often blood samples are obtained.
Talk to a specialist before providing your PML-RARA (APML) test sample to a technician.
What are the parameters measured in PML-RARA (APML)?
In the PML-RARA (APML) test, the presence of the PML-RARA gene or its transcripts is detected. The transcripts from the fusion gene can be detected with the help of PCR-based assays.
Preparations for PML-RARA (APML)
No specific preparations are required for the PML-RARA (APML) test.
Post Care after PML-RARA (APML)
No post-care methods are required after the PML-RARA (APML) test.
Sample Types in PML-RARA (APML)
A blood sample or a bone marrow sample are required in the PML-RARA (APML) test.
Side effects of PML-RARA (APML)
No side effects are observed in the PML-RARA (APML) test.