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Periodic Acid Schiff Stain, Urine

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Overview

The Periodic Acid Schiff (PAS) stain test is performed to highlight glycogen, glycoproteins, glycolipids, and mucins in samples like urine. It can be used to detect Glycogen Storage Diseases.

What is Periodic Acid Schiff Stain Test for Urine?

The Periodic Acid Schiff stain test is a technique used to highlight molecules with a high percentage of carbohydrate content such as glycogen

PAS staining first exposes the sample to periodic acid. Periodic acid acts as an oxidizing agent which oxidizes certain compounds, resulting in dialdehydes. These dialdehydes, when exposed to Schiff’s reagent, forms an insoluble magenta colored complex.

A suitable basic stain is often used as a counterstain. Positive staining for PAS indicates the presence of polysaccharides like glycogen.

Why is Periodic Acid Schiff Stain for Urine Suggested?

Periodic Acid Schiff stain test is often used to detect:

  • Glycogen storage diseases.
  • Adenocarcinoma which often secretes mucin.
  • Fungal infections.

How is Periodic Acid Schiff Stain for Urine Performed?

Periodic Acid Schiff stain test is performed by collecting the patient’s urine sample.

What are the Parameters Measured in Periodic Acid Schiff Stain for Urine?

Periodic Acid Schiff stain test for urine can highlight a high percentage of carbohydrate content, like glycogen, in the urine sample.  

Preparation before Performing Periodic Acid Schiff Stain for Urine

No special preparation is necessary for PAS stain test.

Post-Care after Periodic Acid Schiff Stain for Urine

No post-care is necessary after the PAS stain test.

Sample Types in Periodic Acid Schiff Stain for Urine

Patient’s urine sample is collected for PAS stain test based on the doctor’s recommendations.

Side Effects/Risks of Periodic Acid Schiff Stain for Urine

There are no risks associated with the PAS stain test.

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