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Terminal Deoxynucleotidyl Transferase

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What is Terminal Deoxynucleotidyl Transferase? 12

Terminal Deoxynucleotidyl Transferase (TdT) is a marker used to diagnose leukaemia (blood cancer). TdT is a template independent polymerase that catalyzes the addition of deoxynucleotides to the 3′ hydroxyl terminus of DNA molecules.

Why is Terminal Deoxynucleotidyl Transferase suggested? 4

Common symptoms of leukaemia are:

  • Persistent weakness.
  • Unexpected weight loss.
  • Frequent infections.
  • Swollen lymph nodes.
  • Bone pain.
  • Fever.
  • Easy bleeding or bruising.

How is Terminal Deoxynucleotidyl Transferase performed?3

TdT test is a blood test. The nurse/lab technician rinses the skin with an alcohol pad, inserts a needle into a vein on the rinsed skin, draws the blood and stores it in a small vial. You may feel a slight pinch. The nurse will place a dressing strip on the skin immediately after the process.

The blood sample is sent to the laboratory for diagnosis.

What are the parameters measured in Terminal Deoxynucleotidyl Transferase?1

TdT is used to diagnose lymphoblastic lymphoma, acute myeloid leukaemia or leukaemia.

TdT positive cells can be found in small numbers in the healthy lymph nodes of tonsils or in the malignant cells of acute lymphoblastic leukaemia, and hence this test can help to distinguish between the actual diseases and malignant tumours.

Preparation before performing Terminal Deoxynucleotidyl Transferase 13

There is no fasting required before the test, unless stated by the doctor.

Your doctor might ask you to stop taking any medicines before the test as they may interfere with the test result. He/she will also ask about your lifestyle, family history etc. Consult your doctor before taking any medicines before the test.

Post-care after Terminal Deoxynucleotidyl Transferase 3

Do not remove the dressing strip before 30 mins-1 hour. Avoid any strenuous activity throughout the day. If bleeding occurs, apply pressure with your fingers at the needle site. Avoid wearing tight clothes on your arms.

Sample types in Terminal Deoxynucleotidyl Transferase 1

Blood sample.

Side effects/risks of Terminal Deoxynucleotidyl Transferase 3

Side effects of the blood test might include:

  • Bleeding.
  • Discomfort.
  • Swelling.
  • Slight bruising or throbbing on the needle site.

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