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Stain Albert

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Diphtheria or whooping cough was one of the major causes of death in early childhood during the Twentieth Century. Vaccination and methods of early detection of the disease have brought down the mortality rate considerably and Albert’s Stain is vital in the process of detection of Diphtheria.

What is Albert’s stain?

Albert’s stain is a staining method used to stain Corynebacterium diphtheriae[1] which is the main causative agent for Diphtheria or more commonly known as whooping cough. It is a chronic coughing condition common in children who have not received the DPT vaccination. It may lead to severe complications like neurological deficits and heart diseases.

 

Why is Albert’s stain suggested?

Albert’s stain is suggested if you have the following symptoms:

  • Severe bouts of coughing
  • Swelling of lymph nodes around the neck
  • High fever (101℉ +)

How is Albert’s stain performed?

Albert’s stain testing is fairly simple as it requires:

  • Taking the sputum sample by spitting in a cup (preferably early morning)
  • The sample is processed in a lab by putting Albert’s Stain and observing it under a microscope

What are the parameters measured in Albert’s stain?

In Albert’s stain is specific for testing the number of Corynebacterium diphtheriae (Bacteria) involves the following findings:

  • Presence of green colored[1] bacteria and black granules at one end in the shape of a rod arranged at an angle to each other is considered a positive sign.

 

Preparation before performing Albert’s stain

Albert’s stain testing should be preceded by a little preparation which includes not consuming any food before collecting the sample, hence early morning samples are preferred.

Post-care after Albert’s stain

It’s a simple sputum test that requires no post-care.

Sample types in Albert’s stain

Albert’s stain testing requires taking a sputum sample which is provided by the patient in leak-proof containers to prevent loss of sample or contamination by other microbes.

 

Side effects/ risks of Albert’s stain

Albert’s stain testing involves no risk factors.

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