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Auramine Rhodamine, Urine

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Overview

Auramine rhodamine urine staining is a diagnostic test to identify mycobacteria in the blood.

Whats is  Auramine rhodamine urine staining?

Auramine rhodamine urine staining is used to identify Acid Fast Bacilli (AFB). Auramine rhodamine urine staining is also known as “Truant method of staining”. Auramine rhodamine urine staining test makes use of auramine and rhodamine fluorochrome dyes that bind to mycolic acid. 

Why is Auramine rhodamine urine staining suggested?

Auramine rhodamine urine staining is suggested if the patient is suspected to have a mycobacterial infection.

  • Lack of appetite.
  • Chills and Night Sweats. 

How is Auramine rhodamine urine staining test performed?

Auramine rhodamine urine staining is carried out in a medical facility.

  • A smear is made by suspending the specimen in distilled water.
  • The smear is placed on a slide and is flooded by auramine rhodamine and fluorescent decolorizer.
  • Potassium permanganate is added and is then rinsed with distilled water.

The slide is viewed under a microscope to identify the change in color to confirm the presence of Mycobacterium using Auramine rhodamine urine staining. [2]

What are the parameters measured in the Auramine rhodamine urine staining test?

Auramine rhodamine urine staining is used to identify the change in color of the specimen.

Preparation before performing Auramine rhodamine urine staining

No special preparation is needed before the Auramine rhodamine urine staining test.

  • Check with your doctor to know if there is any specific preparation involved before Auramine rhodamine urine staining.

Post-care after Auramine rhodamine urine staining

No special care is needed after Auramine rhodamine urine staining test.

  • You can resume your normal activities after Auramine rhodamine urine staining.

Sample types in Auramine rhodamine urine staining

Sputum or Phlegm sample is used from Auramine rhodamine urine staining test.

Risks of Auramine rhodamine urine staining test

Auramine rhodamine urine staining test comes with a few risks

  • Auramine and rhodamine are carcinogen agents.
  • Potassium permanganate can cause irritation to skin and eyes. 

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