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Anti ds-DNA Antibody-IFA

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Overview Brief

The Anti ds-DNA Antibody-IFA test is performed using the Indirect Fluorescent Antibody (IFA) method.

What is the Anti ds-DNA Antibody-IFA Test?

Anti ds-DNA Antibody-IFA is a simple blood test to detect the presence of autoantibodies in the blood. Anti ds-DNA Antibody-IFA is one of the most effective tools to diagnose SLE, an autoimmune disorder.

Why is the Anti ds-DNA Antibody-IFA Test Suggested?

Anti ds-DNA Antibody-IFA Test is suggested to confirm SLE and distinguish between SLE and other autoimmune disorders. Symptoms of patients:

  • Pain and inflammation of joints.
  • Presence of the red butterfly rash on the nose and cheeks.
  • Skin sensitivity to light.
  • Tingling of hands and feet.

How is the Anti ds-DNA Antibody-IFA Test Performed?

The Anti ds-DNA Antibody-IFA test is performed using the IFA method.

  • An intravenous blood sample is drawn.
  • The sample is mixed with cells that are affixed on a slide.
  • Autoantibodies in the blood react with the cells on the slide.
  • The slide is treated with an IFA reagent and examined under the microscope.

The results of the Anti ds-DNA Antibody-IFA test are reported as titre, which is a ratio.

What are the Parameters Measured in Anti ds-DNA Antibody-IFA Test?

Anti ds-DNA Antibody-IFA test detects and measures antinuclear antibodies, that target the genetic material in the nucleus of the 

Preparation Before Performing the Anti ds-DNA Antibody-IFA Test

There is no extensive preparation required before taking the Anti ds-DNA Antibody-IFA Test.

  • Wear half sleeves or sleeveless clothes as the sample for Anti ds-DNA Antibody-IFA test is drawn from the arm.
  • Follow doctors’ advice about food and medication before the test.

Post-care After the Anti ds-DNA Antibody-IFA Test

Giving sample for the Anti ds-DNA Antibody-IFA test takes around 10 minutes.

  • Patients can go home and resume their normal routines.
  • Patients are advised to take rest for some time if they feel weak or dizzy.

Sample Types in Anti ds-DNA Antibody-IFA Test

There is only one sample requirement for Anti ds-DNA Antibody-IFA Test: a blood sample.

Side Effects/Risks of the Anti ds-DNA Antibody-IFA Test

The patient has to give a blood sample for the Anti ds-DNA Antibody-IFA test. Risks may include

  • Excessive bleeding.
  • Feeling dizzy.

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